All Sugar Based Cellulose Derivatives Synthesized by Azide–Alkyne Click Chemistry. Click Chemistry Reagents Introduction Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure.1-4 The two-step click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne (Figure 1). Click Chemistry Click Chemistry has been widely used in bioconjugation, biolabeling and material sciences in pharmaceutical and biotech industry due to its mild condition and high selectivity. Search for more papers by this author. This new "Click reaction", unlike conventional Click Chemistry, is very fast at room temperature and does not require a used click chemistry to modify the surface of cellulose .
Due to the easy introduction of azide functional groups and high reliability of the HDC reaction towards the bulkiest of reactants, Liebert et al.

Chih‐Ying Chien. Current Protocols in Chemical Biology 2011, 3 (4) , 153-162. Nicholas Stephanopoulos, Matthew B Francis. "Click chemistry" usually refers to the reaction of an azide with an alkyne yielding 1,5-disubstituted 1,2,3-triazoles. The Click-iT DIBO alkyne reagents (Molecular Probes azide and alkyne derivatives—Table 3.1) are compatible with copper-free click chemistry reactions that capture or detect azide-substituted targets, including metabolic analogs of proteins and many of their …
Choosing an effective protein bioconjugation strategy.

However, in 2002 a copper(I)-catalyzed version was reported independently by Meldal and Sharpless.

DOI: 10.1002/9780470559277.ch110148. We describe herein the synthesis of functional gold nanorods suitable for carrying out “click” chemistry reactions. Copper‐Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation. Without additives, this reaction (Huisgen 1,3-dipolar cycloaddition) is relatively slow. The novel Copper-free Click Chemistry is based on the reaction of a diarylcyclooctyne moiety (DBCO, or ADIBO) with an azide-labeled reaction partner, known as strain-promoted alkyne azide cycloaddition (SPAAC). Center of Excellence for Polysaccharide Research, Institute for Organic Chemistry and Macromolecular Chemistry, Friedrich‐Schiller University of Jena, Humboldtstraße 10, 07743 Jena, Germany . Gold nanorods modified with a copolymer containing sulfonate and maleic acid groups have been conjugated to a bifunctional azide molecule (amine−PEG−azide). Andreas Koschella. Click chemistry is a concept in which modular synthesis is used to rapidly find new molecules with desirable properties1.


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